AgrobacTransV1, Main, Exploration, bibRecord, 000238

Establishment of an In Vivo ARGONAUTE Reporter System in Plants.

Identifieur interne : 000238 ( Main/Exploration ); précédent : 000237; suivant : 000239

Establishment of an In Vivo ARGONAUTE Reporter System in Plants.

Auteurs : Károly Fátyol [Hongrie] ; J Zsef Burgyán [Hongrie]

Source :

Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.

RBID : pubmed:28608335

Descripteurs français

KwdFr :
- ADN complémentaire (génétique), ARN des plantes (génétique), Agrobacterium (génétique), Clonage moléculaire (méthodes), Modèles moléculaires (MeSH), Protéines Argonaute (analyse), Protéines Argonaute (génétique), Protéines végétales (analyse), Protéines végétales (génétique), Régions 3' non traduites (MeSH), Tabac (génétique), Technique de Western (méthodes), Transformation génétique (MeSH), Vecteurs génétiques (génétique), microARN (génétique), Électrophorèse sur gel de polyacrylamide (méthodes).
MESH :
- analyse : Protéines Argonaute, Protéines végétales.
- génétique : ADN complémentaire, ARN des plantes, Agrobacterium, Protéines Argonaute, Protéines végétales, Tabac, Vecteurs génétiques, microARN.
- méthodes : Clonage moléculaire, Technique de Western, Électrophorèse sur gel de polyacrylamide.
- Modèles moléculaires, Régions 3' non traduites, Transformation génétique.

English descriptors

KwdEn :
- 3' Untranslated Regions (MeSH), Agrobacterium (genetics), Argonaute Proteins (analysis), Argonaute Proteins (genetics), Blotting, Western (methods), Cloning, Molecular (methods), DNA, Complementary (genetics), Electrophoresis, Polyacrylamide Gel (methods), Genetic Vectors (genetics), MicroRNAs (genetics), Models, Molecular (MeSH), Plant Proteins (analysis), Plant Proteins (genetics), RNA, Plant (genetics), Tobacco (genetics), Transformation, Genetic (MeSH).
MESH :
- chemical , analysis : Argonaute Proteins, Plant Proteins.
- chemical , genetics : Argonaute Proteins, DNA, Complementary, MicroRNAs, Plant Proteins, RNA, Plant.
- chemical : 3' Untranslated Regions.
- genetics : Agrobacterium, Genetic Vectors, Tobacco.
- methods : Blotting, Western, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel.
- Models, Molecular, Transformation, Genetic.

Abstract

RNA silencing is not only an evolutionarily conserved gene regulatory mechanism, but in plants also serves as the basis for robust adaptive antiviral immune responses. ARGONAUTE (AGO) proteins form the catalytic cores of the RNA-guided ribonuclease complexes, which play a central role in RNA silencing. Here we describe an in vivo assay system for analyzing the activities of AGO proteins in the virological model plant Nicotiana benthamiana .

DOI: 10.1007/978-1-4939-7165-7_5
PubMed: 28608335

Affiliations:

Hongrie

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Fatyol, Karoly" sort="Fatyol, Karoly" uniqKey="Fatyol K" first="Károly" last="Fátyol">Károly Fátyol</name>
<affiliation wicri:level="1"><nlm:affiliation>Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, Szent Györgyi Albert u. 4., Gödöllő, 2100, Hungary. kfatyol@gmail.com.</nlm:affiliation>
<country xml:lang="fr">Hongrie</country>
<wicri:regionArea>Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, Szent Györgyi Albert u. 4., Gödöllő, 2100</wicri:regionArea>
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<author><name sortKey="Burgyan, J Zsef" sort="Burgyan, J Zsef" uniqKey="Burgyan J" first="J Zsef" last="Burgyán">J Zsef Burgyán</name>
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<term>Argonaute Proteins (genetics)</term>
<term>Blotting, Western (methods)</term>
<term>Cloning, Molecular (methods)</term>
<term>DNA, Complementary (genetics)</term>
<term>Electrophoresis, Polyacrylamide Gel (methods)</term>
<term>Genetic Vectors (genetics)</term>
<term>MicroRNAs (genetics)</term>
<term>Models, Molecular (MeSH)</term>
<term>Plant Proteins (analysis)</term>
<term>Plant Proteins (genetics)</term>
<term>RNA, Plant (genetics)</term>
<term>Tobacco (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term>
<term>ARN des plantes (génétique)</term>
<term>Agrobacterium (génétique)</term>
<term>Clonage moléculaire (méthodes)</term>
<term>Modèles moléculaires (MeSH)</term>
<term>Protéines Argonaute (analyse)</term>
<term>Protéines Argonaute (génétique)</term>
<term>Protéines végétales (analyse)</term>
<term>Protéines végétales (génétique)</term>
<term>Régions 3' non traduites (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Technique de Western (méthodes)</term>
<term>Transformation génétique (MeSH)</term>
<term>Vecteurs génétiques (génétique)</term>
<term>microARN (génétique)</term>
<term>Électrophorèse sur gel de polyacrylamide (méthodes)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Argonaute Proteins</term>
<term>Plant Proteins</term>
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<term>DNA, Complementary</term>
<term>MicroRNAs</term>
<term>Plant Proteins</term>
<term>RNA, Plant</term>
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<term>Protéines végétales</term>
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<term>Protéines végétales</term>
<term>Tabac</term>
<term>Vecteurs génétiques</term>
<term>microARN</term>
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<term>Cloning, Molecular</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
</keywords>
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<term>Technique de Western</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Models, Molecular</term>
<term>Transformation, Genetic</term>
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<term>Régions 3' non traduites</term>
<term>Transformation génétique</term>
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<front><div type="abstract" xml:lang="en">RNA silencing is not only an evolutionarily conserved gene regulatory mechanism, but in plants also serves as the basis for robust adaptive antiviral immune responses. ARGONAUTE (AGO) proteins form the catalytic cores of the RNA-guided ribonuclease complexes, which play a central role in RNA silencing. Here we describe an in vivo assay system for analyzing the activities of AGO proteins in the virological model plant Nicotiana benthamiana .</div>
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<Month>03</Month>
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<Month>07</Month>
<Day>09</Day>
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<Title>Methods in molecular biology (Clifton, N.J.)</Title>
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<ArticleTitle>Establishment of an In Vivo ARGONAUTE Reporter System in Plants.</ArticleTitle>
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	Serveur d'exploration sur l'agrobacterium et la transgénèse
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Serveur d'exploration sur l'agrobacterium et la transgénèse

Establishment of an In Vivo ARGONAUTE Reporter System in Plants.

Establishment of an In Vivo ARGONAUTE Reporter System in Plants.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki